 | Samuel Freeman Ayer (1840-1899) 
|
 | America E. Evans (1841-1920) |
 | Frank Milton Ayer (1863-1918) |
| Edith E. Ayer (1865-1951) |
| Henry Melville Ayer (1866-1935)  |
| Ethel C Ayer (1868-1954) |
| Florence Elizabeth Ayer (1875-1956) |
| Helen Ayer (1882-1970) |
| Josiah E. Ayer (1883-1954) |
| Samuel Elden Ayer (1885-1970)
|
| Beatrice J. Johnson (1881-1958) |
 | Constance June Ayer (1911-1996) |
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